Montescudaio is a comune (municipality) in the Province of Pisa in the Italian region Tuscany, located about 70 kilometres (43 mi) southwest of Florence and about 45 kilometres (28 mi) southeast of Pisa.
Castril, Spain, since 2006
Eberstadt, Germany, since 1984
Charlotte Amalie, U.S. Virgin Islands, since 2010
Christiansted, U.S. Virgin Islands, since 2010
Frederiksted, U.S. Virgin Islands, since 2010